Are you interested in learning more about In Vitro Fertilization (IVF)? La Jolla IVF has put together a protocol and process guide for prospective parents. Check it out today and see if IVF is right for you.

Created By: Saira Date: 04.10.2019

In vitro fertilization (IVF) is a process of fertilization where an egg is combined with sperm outside the body, in vitro. The process involves monitoring and stimulating a woman’s ovulatory process, removing an ovum or ova (egg or eggs) from the woman’s ovaries and letting sperm fertilize them in a liquid in a laboratory. After the fertilized egg (zygote) undergoes embryo culture for 2–6 days, it is implanted in the same or another woman’s uterus, in a procedure called embryo transfer.

IVF is a type of assisted reproductive technology used for infertility treatment and gestational surrogacy. A fertilized egg may be implanted into a surrogate’s uterus, and the resulting child is genetically related to the surrogate, who is the birth mother, but not to the intended parent.


IVF begins with controlled ovarian hyperstimulation (COH), using gonadotropins, to induce ovulation in women who do not ovulate or ovulate irregularly. Next, the ovulated eggs are retrieved from the woman’s ovaries using a transvaginal ultrasound-guided needle piercing the vaginal wall.

The retrieved eggs are fertilized by sperm in a laboratory, in a process called insemination, using one of the following methods:

  • Intracytoplasmic sperm injection (ICSI): A single sperm is injected into each egg to fertilize it.
  • Conventional insemination: Sperm is placed in a dish containing the eggs, and fertilization occurs as the sperm “swim” up into the eggs.

After fertilization, the eggs are incubated for 5–6 days in a culture medium in order to allow embryo cleavage to occur.

embryo transfer

Embryo transfer is the process of placing a developed embryo or embryos into the uterus of a female patient, for the purpose of establishing a pregnancy.

Embryo transfer is typically performed 3-5 days after egg retrieval, when the embryo(s) have reached the blastocyst stage of development.

Prior to embryo transfer, the embryos are graded on a scale of 1-5, based on their appearance and development.

Embryos that are grade 1 or 2 are considered to be the best quality and have the highest chance of implantation and pregnancy.

Embryos that are grade 3 or 4 are considered to be of good quality and have a decent chance of implantation and pregnancy.

Embryos that are grade 5 are considered to be of poor quality and have a low chance of implantation and pregnancy.

During embryo transfer, a catheter is inserted through the cervix into the uterus. The embryo(s) are then injected through the catheter into the uterus.

Embryo transfer is usually a painless procedure that takes about 5-10 minutes to perform.

After embryo transfer, the patient is usually asked to

In in vitro fertilization we use medications to encourage a woman’s ovaries to grow and develop. We use vaginal probe ultrasound as well as blood hormonal levels to make sure that her body is responding appropriately. When the lead follicles reach about eighteen to twenty millimeters in diameter under ultrasound guidance under anesthesia I stick a needle through the vagina into the ovary and in a simple 15 minute procedure surgically remove eggs.

Those eggs are placed in a petri dish and then the gentleman sperm is evaluated and there are two approaches one can take a conventional IVF approach where 50,000 sperm are placed on top of each egg and then we rely on the sperms ability to penetrate the egg and the next day we know you have embryos.

Or more commonly we use a procedure called ICSI intracytoplasmic sperm injection we’re under the microscope we hold a single egg and we insert a single sperm into each egg under the microscope and the insertion of the sperm occurs into the cytoplasm in the egg and this is called ICSI or intracytoplasmic sperm injection.

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